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第1篇 化学教育 3

1-1 化学专业课程体系设计 3

1-2 北京大学化学基地建设的实践与体会 7

1-3　分析化学教学改革与人才培养 12

第2篇 综述 17

2-1　分析化学概述 17

2-2　某些滥用药物分析的进展 26

2-3　药物免疫分析及其进展 35

2-4　稀土螯合物探针及其在时间分辨荧光免疫分析中的应用 44

2-5　脂质体放大免疫分析的进展 51

2-6　NIPA系温度敏感水凝胶及分析应用 59

2-7　小分子发光免疫分析及其进展 66

2-8　胶体金免疫分析方法的进展 82

2-9　蛋白芯片研究进展 90

2-10　酚类环境雌激素的分析研究进展 96

2-11　EPO检测技术的研究进展 106

2-12　兴奋剂——禁用物质及禁用手段简介 115

2-13　兴奋剂检测与分析化学的发展 123

2-14　中药指纹图谱技术 131

2-15　生化探针技术 139

第3篇 研究论文 151

3-1 催化显色反应的研究——V.Nb(Ⅴ),Ta(Ⅴ)-邻氨基苯酚-过氧化氢-酒石酸体系 151

3-2　分光光度法测定金属铟中痕量铝 156

3-3　4,4′-二（二乙氨基）苯硫酮和Triton X-100分光光度测定钯 159

3-4　利用4,4′-二（二乙氨基）苯硫酮和Triton X-100分光光度法测定银 165

3-5　铂（Ⅱ）与4,4′-双（二甲胺）二苯甲硫酮显色反应的研究 170

3-6　痕量铜的催化光度测定 175

3-7　催化显色反应的研究——X．铜（Ⅱ）-没食子酸-过氧化氢-活化剂体系 178

3-8　4,4′-二（二乙氨基）二苯硫酮与铂（Ⅱ）显色反应的研究 183

3-9　4,4′-二（二乙氨基）二苯硫酮分光光度法测定铑 188

3-10　Determination of Platinum(Ⅳ)with Silver(Ⅰ)and 4,4′-Bis(diethylamino)Thiobenzophenone 192

3-11　模拟过氧化物酶用于葡萄糖的测定方法研究 198

3-12　Spectrofluorimetric Determination of Tetracycline and Anhydrotetracycline in Serum and Urine 202

3-13　Fluorimetric Determination of Tetracycline and Anhydrotetracycline 207

3-14　4,4′-二（二乙氨基）二苯硫酮分光光度法测定痕量铜 212

3-15　镧系螯合物荧光免疫分析法测定对硫磷 217

3-16　Preliminary Studies on the Immunochemical Behavior of BSA-TTA-Eu Conjugates 221

3-17　吗啡的化学发光测定方法的研究 224

3-18　Double-label Simultaneous Time-resolved Fluoroimmunoassay of Phenytoin and Phenobarbital 230

3-19　高分子控温相变免疫分析法测定苯妥因 238

3-20　Liposome Immunoassay for Determination of Phenytoin 243

3-21 时间分辨荧光免疫分析法直接测定雌二醇 247

3-22　Thermally Reversible Hydrogel Based Fluoroimmunoassay of Methyltestosterone 253

3-23　甲基睾酮的控温相分离免疫分析 261

3-24　甲基睾酮的控温相分离荧光免疫分析 267

3-25 A New Spectrophotometric Method for the Determination of Aniline in Environmental Water Samples 272

3-26 Thermally Reversible Hydrogel Modified Capillary Electrophoretic Immunoassay of Estradiol with Laser-induced Fluorescence Detection 278

3-27　水杨酸及其氯代物的毛细管电泳分析研究 288

3-28　吗啡的控温相分离胶体金标记免疫分析 292

3-29　时间分辨荧光免疫分析法间接测定雌二醇 298

3-30 A Phase Separation Fluoroimmunoassay of Estradiol 303

3-31　生物素-亲和素体系测定雌酮 312

3-32　Enhancement of the Sensitivity of a Capillary Electrophoresis Immuno-assay for Estradiol with Laser-induced Fluorescence Based on a Fluorescein-labeled Secondary Antibody 319

3-33 Ricin Determination by Temperature Controlled Phase-separation Immunoassay 328

3-34 Spectrophotometric Determination of Aniline in Polluted Surface Water and Wastewater-improved Reagents in Test Kit Format 335

3-35　Production and Characterization of Anti-estrone Monoclonal Antibody 342

3-36　基于温度敏感水凝胶的雌二醇荧光免疫分析 353

3-37　水中苯胺类化合物的分光光度法测定 358

3-38　二元共聚物热解碳包覆的石墨负极材料 363

3-39　雌三醇单克隆抗体的制备与表征 368

3-40　A New Competitive Enzyme-linked Immunosorbent Assay(ELISA)for Determination of Estrogenic Bisphenols 376

3-41　Development and Characterization of an Immunoaffinity Column for the Selective Extraction of Bisphenol A from Serum Samples 384

3-42　控温相变免疫分析测定雌酮 396

3-43　粒度对石墨负极材料嵌锂性能的影响 403

3-44　毛细管电泳免疫分析法分析雌三醇 411

3-45 Rapid and Sensitive Determination of Protein by Light-scattering Technique with Eriochrome Blue Black R 416

3-46　电感耦合等离子发射光谱法测定血清及组织中铂类药物 426

3-47 Simultaneous Separation and Determination of Five Bioactive Components in Traditional Chinese Medicinal Formula,GuanxinⅡ,by HPLC 432

3-48　Direct Immunoassay of Estriol in Pregnancy Serum by Capillary Electropho-resis with Laser-induced Fluorescence Detector 443

3-49　Pharmacokinetics and Tissue Distribution of iv Injection of Polyphase Liposome-encapsulated Cisplatin(KM-1)in Rats 452

3-50　双酚A分子印迹聚合物的制备和识别性能研究 458

3-51 Sensitive ELISA for Determination of Serum E2 Using a New Tracer E2-Biotin 463

3-52　Production and Characterization of Antirecombinant Human Erythropo-ietin(rhEPO)Monoclonal Antibody 473

3-53　减肥保健品中芬氟拉明等8种合成食欲抑制剂的分析测定 481

3-54　反相高效液相色谱法测定葛根素和大豆苷元 487

3-55 Micelle-mediated Extraction of Tanshinones from Salvia Miltiorrhiza Bunge with Analysis by High-performance Liquid Chromatography 492

3-56 RP-HPLC Determination of Octanol-water Partition Coefficients for Bio-active Compounds from Chinese Herbal Medicines 504

3-57　Comparison of Microwave-assisted and Ultrasound-assisted Extraction for Determi-nation of Main Water-soluble Bioactive Constituents in Traditional Chinese Medicinal Preparation Tongmaichongji by HPLC-DAD 516

3-58　酶联免疫分析结合生物素-亲合素放大体系测定血清中的雌三醇 528

3-59　Different Biotinylation Strategies for Competitive Immunoassay of Estradiol 534

3-60 Study of Immunoassay Methods for Recombinant Human Erythro-poietin(rhEPO)Using Competitive ELISA 544

3-61　Simultaneous Separation and Determination of Four Bioactive Constituents in Traditional Chinese Medicinal Tablet Xinkeshu by HPLC-DAD 548

3-62　Determination of Papaverine by Biotin-avidin Amplified ELISA 556

3-63 Biotin-avidin Amplified Enzyme-linked Immunosorbent Assay for Determination of Isoflavone Daidzein 565

3-64 Simultaneous Determination of Cryptotanshinone,Tanshinone Ⅰand TanshinoneⅡ A in Traditional Chinese Medicinal Preparations Containing Radix Salvia Miltiorrhiza by HPLC 575

3-65 Simultaneous Determination of Five Marker Constituents in Traditional Chinese Medicinal Preparation Le-Mai Granule by High Performance Liquid Chromatography 584

3-66 Development of an Indirect Competitive ELISA for the Determination of Papaverine 593

3-67 Analysis of Isoflavone Daidzein in Puerariae radix with Micelle-mediated Extraction and Preconcentration 604

3-68 Isolation and Characterization of an Anti-recombinant Erythropoietin Single-chain Antibody Fragment Using a Phage Display Antibody Library 615

附录 627

附录1　编译著目录 627

附录2 “非放射免疫分析的应用基础研究”工作小结 628

附录3　常文保教授生平 636

编后记 638