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Section A - Cells and macromloecules 1

A1 Cellular Classification 1

A2 Subcellular organelles 4

A3 Macromolecules 7

Abbreviations 9

F4 Recombimation 9

A4 Large macromolecular assemblies 11

Preface to the second edition 11

Preface to the first edition 13

Section B - Protein structure 15

B1 Amino acids 15

B2 Protein structure and function 18

B3 Protein analysis 25

C1 Nucleic acid structure 31

Section C - Properties of nucleic acids 31

C2 Chemical and physical properties of nucleic acids 38

C3 Spectroscopic and thermal properties of nucleic acids 42

C4 DNA supercoiling 45

Section D - Prokaryotic and eukaryotic chromosome structure 49

D1 Prokaryotic chromosome structure 49

D2 Chromatin structure 51

D3 Eukaryotic chromosome structure 56

D4 Genome complexity 61

D5 The flow of gengtic information 66

E1 DNA replication：an overview 71

Section E - DNA replication 71

E2 Bacterial DNA replication 76

E3 The cell cycle 80

E4 Eukaryotic DNA replication 84

Section F- DNA damage,repair and recombimation 89

F1 Mutagenesis 89

F2 DAN damage 93

F3 DAN repair 96

Section G - Gene manipulation 103

G1 DAN cloning：an overview 103

G2 Preparation of plasmid DNA 108

G3 Restriction dnzymes and electriphoresis 111

G4 Ligation.transformation and analysis of recombinants 116

Section H - Cloning vectors 123

H1 Desing of plasmid vectors 123

H2 Bacteriophage vectors 127

H3 Cosmide,YACs and BACs 132

H4 Eukaryotic vectors 137

Section I - Gene libraries and screening 143

I1 Genomic libraries 143

I2 cDNA libraries 146

I3 Screening procedures 151

Section J - Analysis and uses of cloned DNA 155

J1 Charactrization of clones 155

J2 Nucleic acid sequencing 160

J3 Polymeraes chain reaction 165

J4 Organization of clonen genes 170

J5 Mutagenesis of cloned genes 174

J6 Applications of cloning 178

Section K - Transcription in prokaryotes 183

K1 Basic principles of transcription 183

K2 Escherichia coli RNA poly merase 186

K3 The E. coli a 70 promoter 188

K4 Transcription,initiation,elongation and etrmination 191

Section L - Regulation of transcrlption in prokaryotes 197

L1 The lac operon 197

L2 The trp operon 201

L3 Transcriptional regulation by alternative or factors 205

Section M - Transcription in eukaryotes 209

M1 The three RNA polymerases:characterization and function 209

M2 RNA polⅠ genes:the ribosomal repeat 211

M3 RNA pol Ⅲ genes： 5S and tRNA transcription 215

M4 RAN PolⅡgenes;promoters and enhancers 219

M5 General transcripton factors and RNA Pol Ⅱinitiation 221

Section N - Regulation of transcription in eukaryotes 225

N1 Eukaryotic transcription facrots 225

N2 Examples of transcriptional regulation 231

Section O - RNA processing and RNPs 237

O1 rRNA processing and ribosornes 237

O2 tRNA processing,RNase P and ribozymes 243

O3 mRNA processing, hnRNPs and snRNPs 246

O4 Alternative mRNA processing 252

Section P - The genetic code and tRNA 255

P1 The genetic code 255

P2 tRNA structuer and function 259

Section Q - Protenin symthesis 265

Q1 Aspects of protein symthesis 265

Q2 Mechanism of protein symthesis 269

Q3 Initiation in eukaryotes 275

Q4 Translational control and post-translational events 279

R1 Introduction to viruese 283

Section R - Baclexiophages and eukaryotic viruses 283

R2 Bacterophages 287

R3 DNA viruses 292

R4 RNA viruses 296

Section S - Tumor viruses and oncogenes 301

S1 Oncogenes found in turnor viruses 301

S2 Categories of on cogenes 305

S3 Tumor suppressor genes 308

S4 Apoptosis 312

Futrher reading 317

Multiple choice questions 321

Ansers 337

Index 339