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Chapter 1. An Introduction of Functional Experimentation 1

Chapter 2. Basic Knowledge and Theory 7

Section 1. Biological Characteristics and Application of Commonly Used Laboratory Animals 7

Section 2. Biological Data Acquisition Systems 13

Section 3. Selection and Design Principles of Medical Scientific Research Topics 20

Section 4. Medical Information Retrieval 27

Section 5. Writing Methods of Scienti-fic Papers and Lab Reports 30

Section 6. Laboratory Safety and Rules 35

Chapter 3. Basic Methods of Animal Experiments 43

Section 1. Animal Capture and Restraint 43

Section 2. Animal Numbering 46

Section 3. Drug Administration for Laboratory Animals 48

Section 4. Anesthesia of Laboratory Animals 52

Section 5. Unhairing of Laboratory Animals 57

Section 6. Blood Collection of Laboratory Animals 58

Section 7. Euthanasia Methods of Laboratory Animals 65

Chapter 4. Basic Techniques in Experimental Animal Surgical Operation 67

Section 1. Traditional Surgical Instruments 67

Section 2. Technique of Animal Catheteriz ation 69

Section 3. Haemostasis and Technique of Knots Making 72

Section 4. Technique of Wound Sutures （Stitches）and Sutures Out 74

Chapter 5. Basic Experiments 76

Experiment 1. The Nature of Muscle Contraction 76

Experiment 2. Measurement of Conduction Velocity of Action Potential and Excitability from Neural Trunk 78

Experiment 3. Determination of Hemogl-obin Content and Red Blood Cell Osmotic Fragility 82

Experiment 4. Premature Contraction and Compensatory Pause 84

Experiment 5. Chemicals on the regulation of ex vivo heart activities 86

Experiment 6. Measurement of Human Arterial Blood Pressure 88

Experiment 7. Recording of Human Ele- ctrocardiogram 91

Experiment 8. Pulmonary Function Tests 93

Experiment 9. Regulation of Respiratory Movements 96

Experiment 10. Physiological Properties of the Isolated Gastrointes- tinal Smoth Muscle 98

Experiment 11. Factors That Influencing Urine Formation 100

Experiment 12. Damage Effects of Unilat eral Labyrinth and Cochlear Potential Induction from Guinea Pigs 103

Experiment 13. Examination of Visual Fields and Scotomas 105

Experiment 14. Analysis of Reflex Arc and the Basic Features of the Reflex Activities 107

Experiment 15. Experimental Pulmonary Edema in Rabbits 111

Experiment 16. Effect of Different Factors on Hypoxic Tolerance 113

Experiment 17. Influence of pH of Body Fluids on Drug Absorption 114

Experiment 18. One-Compartment Open Model and Calculation of the Pharmacokinetic Parameters 115

Experiment 19. Two-Compartment Open Model and Calculation of the Pharmacokinetic Para-meters 119

Experiment 20.Determination of Medial Lethal Dose(LD50)of Drug 122

Experiment 21.The Antergic Experiments of Drug—the Binding Assay of Radio-Ligand and Receptor 123

Experiment 22.Intoxication and Detoxication of Organophosphates 126

Experiment 23.Effects of Drugs by Differ-ent Dosage Forms 128

Experiment 24.Effects of Drugs by Differ-ent Routes of Administrat-ion 130

Experiment 25.Basic Effects of Drugs 131

Experiment 26.Factors Influencing Drug Effects 133

Experiment 27.Observation of Analgesic Action of Drugs with Wri; thing Method 136

Experiment 28.Anticonvulsant Effect of the Drug 137

Experiment 29.Effects of Drugs on Lear- ning andMemory ofAnimal (Mice step-down experiment, rat shuttle box expenment, morris watermaze experiment) 139

Experiment 30.Effects of Hydrocortisone on Mice Ear Swelling In- duced by Xylol 144

Experiment 31. Anti inflammatory Effects of Dexamethasone on Rat Paw Edema 145

Experiment 32. Effects of Cardiac Glycosides on Heart Failure 147

Experiment 33. Antibacterial Experiment of Antibacterial Agents in Vitro 150

Experiment 34. Antibacterial Experiment of Antibacterial Agents in Vivo 152

Chapter 6. Comprehensive Experiments 157

Experiment 1. Different Types of Hypoxia 157

Experiment 2. Hyperkalemia in Rabbits 159

Experiment 3. Disseminated Intravascular Coagulation in Rabbit 161

Experiment 4. Disorders of Acid-Base Bal-ance and Its Experimental Therapy in Rabbits 166

Experiment 5. Ischemia and Reperfusion Injury in Intestine 170

Experiment 6. Acute Endotoxic Shoek 172

Experiment 7. Hemorrhagic Shock and Ah-erations of Microcirculation in Rabbit 175

Experiment 8. Regulation of the Cardiovasc-ular System and Acute Card-iac Failure 178

Experiment 9. Effects of Experimental Pn-eumothorax on Respiration, Circulation and Acid-Base Balance in Rabbits 183

Experiment 10. Acute Respiratory Distress Syndrome and Pharmacoth- erapy 186

Experiment 11. Hepatic Encephalopathy and Its Treatment 189

Experiment 12. Determination of Endogen-ous Creatinine Clearance Rate and Excretion Fraction of Sodium Filtration in Acute Renal Failure 192

Experiment 13. Reproduction of Multiple Organ Dysfunction Syndr-ome 193

Experiment 14. The Effect of Drug and Bio-activator on the Isolated Tracheal Strips of Cavy 198

Experiment 15. Determination of the Phar-macokinetic Parameters of Sulfa Drugs in Rabbits with Renal Failure 200

Chapter 7. Exploratory Experiments 204

Chapter 8. Case Discussion and Paper Analysis 205

References 239

Appendix 240